Molecular characterization of class 1 integrons in clinical strains of Salmonella typhimurium isolated in Slovakia.
نویسندگان
چکیده
The presence of class 1 integrons was investigated in 156 epidemiologically unrelated Salmonella Typhimurium isolates. Of these 156 isolates, 70 were of definitive phage type DT104 and 86 were strains of various phage type, RDNC and untypable, designated here as non-DT104 strains. Integrons were found in 47 of DT104 isolates (67.1%), while in all strains with characteristic pentaresistance (R-type ACSSuT) two integrons 1.0 kb and 1.2 kb in size were found. Among 86 non-DT104 strains, integrons with sizes of 1.6 kb and 1.9 kb in four multidrug-resistant strains DT193 and U302 were found. The integrons from selected strains were further sequenced and the aadA1, aadA2, dhfr1, dhfr12 and bla(PSE) genes were found embedded in cassettes.
منابع مشابه
Evaluation of Class 1 and 2 Integrons and Antibiotic Resistance Pattern in Salmonella enterica Isolated from Diarrheal Food-Borne Outbreaks in Iran
Background: Salmonella spp. are major causes of food-borne disease and have been identified among many diarrheal outbreaks. The major aim of the current investigation was to evaluate the class 1 and 2 integrons and antibiotic resistance pattern in Salmonella enterica isolated from diarrheal food-borne outbreaks in Iran. Methods: This study was carried out on 115 diarrheal feces samples obtain...
متن کاملMolecular study of Salmonella typhimurium integrons gene isolated from food sources and the effect of probiotic Bifidobacterium bifidum on its expression by Real time PCR
Background: Salmonella belongs to the family of Enterobacteriaceae, an intestinal, gram negative, and anaerobic bacterium. Salmonella affects a wide range of vertebrates, mainly through direct and indirect contact with the infected source. Salmonellosis is a dangerous infectious disease and a common human-animal disease. The integron (int) gene is effective in drug resistance and bacterial prol...
متن کاملComparative phenotypic and genotypic characterization of temporally related nontyphoidal Salmonella isolated from human clinical cases, pigs, and the environment in North Carolina.
Nontyphoidal Salmonella infections caused by antimicrobial-resistant (AMR) strains are of great public health concern. We compared the phenotypic and genotypic relationships among temporally and spatially related AMR Salmonella isolates (n=1058) representing several predominant serovars, including Salmonella Typhimurium, Salmonella Typhimurium var. 5-, Salmonella Derby, Salmonella Heidelberg, S...
متن کاملبررسی اینتگرون کلاس 1 و 2 و 3 در سویه های سالمونلا انتریتیدیس بالینی با روش PCR
Background & Objective: Gastroenteritis is one of the most common Salmonella infections in human which is caused by Salmonella serotypes especially S.enteritidis and S.typhimurium. The spread of multi-drug resistant (MDR) Salmonella strains is a serious global issue. Obtaining integrons is considered as one of the most important factors in multi-drug resistance among gram-negative microorganism...
متن کاملCharacterization of drug resistant Salmonella enterica serotype Typhimurium by antibiograms, plasmids, integrons, resistance genes and PFGE.
Forty-seven Salmonella Typhimurium (33 zoonotic, 14 clinical) strains were tested for antimicrobial resistance using the standard disk diffusion method. Presence of relevant resistance genes and class 1 integrons were investigated by using PCR. Pulsed-field gel electrophoresis (PFGE) and plasmid profiling were carried out to determine the genomic diversity of Salmonella Typhimurium. Approximate...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Polish journal of microbiology
دوره 56 1 شماره
صفحات -
تاریخ انتشار 2007